From kiss-and-run to crosstalk

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"Kiss and run" exocytosis at hippocampal synapses.

We have combined electrophysiology and imaging to measure the release of neurotransmitter and fluorescent dye at synapses of cultured hippocampal neurons. These experiments have revealed a "kiss and run" mode of exocytosis in which synaptic vesicles release glutamate normally but do not permit dye to enter or escape from the vesicle. During "kiss and run," the vesicle interior may be exposed ve...

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Secretion: Kiss and Run Caught on Film

Recent results have provided graphic support for the hypothesis that vesicle secretion involves a 'kiss-and-run' mechanism. Evanescent field microscopy has shown that, during exocytosis, intravesicular markers escape without collapse of the vesicular membrane into the surface membrane and that the empty vesicle is immediately retrieved back into the cell.

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The Itinerary of Autophagosomes: From Peripheral Formation to Kiss-and-Run Fusion with Lysosomes

Macroautophagy, a constitutive process in higher eukaryotic cells, mediates degradation of many long-lived proteins and organelles. The actual events occurring during the process in the dynamic system of a living cell have never been thoroughly investigated. We aimed to develop a live-cell assay in which to follow the complete itinerary of an autophagosome. Our experiments show that autophagoso...

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Secretion: Dense-core vesicles can kiss-and-run too

New measurements show that the entire transmitter contents of a dense-core vesicle can be released within a second through a narrow fusion pore that opens transiently. With other results, this raises the possibility that some dense core vesicles may, like small synaptic vesicles, undergo immediate recycling.

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Kiss-and-run, collapse and 'readily retrievable' vesicles.

Two models of synaptic vesicle recycling have been intensely debated for decades: kiss-and-run, in which the vesicle opens and closes transiently, presumably through a small fusion pore, and full fusion, in which the vesicle collapses into the plasma membrane and is retrieved by clathrin-coat-dependent processes. Conceptually, it seems that kiss-and-run would be faster and would retrieve vesicl...

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ژورنال

عنوان ژورنال: Nature Reviews Neuroscience

سال: 2005

ISSN: 1471-003X,1471-0048

DOI: 10.1038/nrn1815